Surgical site infections (SSIs) represent a significant burden on healthcare systems globally, contributing to increased patient morbidity, mortality, prolonged hospital stays, and substantial economic costs. Understanding the local bacteriological profile of aerobic isolates from SSIs and their antimicrobial susceptibility patterns (antibiogram) is crucial for guiding empirical antibiotic therapy, implementing effective infection control measures, and combating the rising threat of antimicrobial resistance. This clinical study aimed to identify the predominant aerobic bacterial pathogens causing SSIs in a tertiary care hospital and to determine their susceptibility to commonly used antibiotics. The findings reveal a diverse spectrum of aerobic bacteria, with a notable prevalence of Gram-negative organisms exhibiting concerning levels of resistance to multiple antimicrobial agents. These insights are vital for informing institutional antibiotic policies, enhancing surveillance strategies, and ultimately improving patient outcomes by facilitating targeted and effective treatment of SSIs.