Volume 7.
Issue 05.

Ferula moschata (syn. Ferula sumbul) is a medicinal plant traditionally used in Central Asian and Middle Eastern folk medicine for its stimulant, neuroprotective, and anti-inflammatory properties. Recent studies have highlighted its potential as a source of bioactive compounds with significant pharmacological effects. This study investigates the antioxidant and anti-inflammatory properties of Ferula moschata extract through a comprehensive phytochemical and biochemical approach.

Using high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS), we identified key bioactive constituents, including coumarins, terpenoids, and phenolic compounds, which contribute to its therapeutic potential. In vitro antioxidant assays (DPPH, FRAP, and ABTS) demonstrated strong free radical scavenging activity, comparable to standard antioxidants such as ascorbic acid and Trolox. Additionally, the extract exhibited dose-dependent inhibition of pro-inflammatory mediators (NO, TNF-α, and IL-6) in lipopolysaccharide (LPS)-induced macrophage cells, suggesting potent anti-inflammatory effects.

Molecular docking studies further revealed interactions between Ferula moschata compounds and key inflammatory markers (COX-2, iNOS), supporting its mechanism of action. These findings provide a scientific basis for the traditional use of Ferula moschata in oxidative stress and inflammation-related disorders, suggesting its potential as a natural therapeutic agent. Further in vivo studies are warranted to validate its efficacy and safety for clinical applications.

Multiseptate gallbladder is a congenital alteration in the formation of this organ, resulting in septa in the lumen of the gallbladder. Cases of patients with this condition is quite rare in the world database, which means that the amount of papers published on this subject can be hard to find and in a few numbers. These conditions usually do not lead to symptoms in which a patient can live the entire life without a diagnosis; however, some patients may present an association with another pathology, especially in the abdominal region, which leads to an evaluation of the gallbladder and diagnosis of this septa. The main ways to diagnose this condition are through imaging tests, such as ultrasonography and computed tomography, which allow the perception of the wall, volume, consistence and lumen of this organ. Therefore, the present case report presents a picture of an asymptomatic patient diagnosed with multiseptate gallbladder. In addition, a review of the literature on the similar case reports presents in databases such as PubMed is included. Applying the knowledge acquired from the literature found it’s possible to understand that this condition occurs because of an alteration on the process that forms the gallbladder and it can manifest with symptoms or it can stay asymptomatic and just be found by accident. When this condition creates symptoms, the patient may need to go through a surgical procedure, like a cholecystectomy, but its only necessary in the presence of manifestations, otherwise, the patient can live a normal life with treating this alteration.

This study investigates immunological distinctions between clinical subtypes of autism spectrum disorder (ASD), focusing on pediatric populations diagnosed with Kanner’s and Asperger’s syndromes. Utilizing quantitative analysis of serum cytokines—IL-6, TNF-α, IL-10, and IL-1β—we evaluated immune deviations in relation to behavioral severity and neurocognitive function. The data revealed subtype-specific inflammatory patterns, with heightened IL-6 and TNF-α in Kanner’s syndrome and selective IL-1β elevation in Asperger’s syndrome. A deficiency in IL-10 was common across both groups, suggesting impaired anti-inflammatory modulation. These results underscore the role of immune biomarkers in refining ASD subtypes and tailoring early interventions.

In our article we analyzed literary sources on thyroid diseases.

The article considers the integration of aesthetic practices of nail care with clinical knowledge in order to avoid risks to the health of the nails by their growing popularity within the beauty industry. This paper identifies the principal issues connected with modern practices such as gel polishes, acrylic extensions, and nail art and argues for an interdisciplinary concept combining Dermatology and Aesthetics. The relevance is based on how fast the market for nail services is growing, and more complications such as onycholysis allergic reactions and infections are due to improperly performed procedures. The scientific novelty consists of a systematic analysis of aesthetic tendencies and clinical risks that lead to recommendations for safe practice. This was gleaned from analyzing eight sources comprising market reports, clinical studies, and technology reviews. The key results show that the addition of clinical knowledge to nail beauty requires; 1) the creation of strict guidelines for instrument sterilization; 2) training of practitioners in safe practices, thinking about the structure of the nail part; 3) developing composite materials with reduced toxicity (for example, acrylates). It has been found that even "soft" ways, like taking off gel polish, make microcracks if done badly. Customers need to pick licensed shops that follow clean rules and do not use fake coats in a way that stops the nail plate from getting thin. The outlook for the industry’s growth lies in dermatologists and manicurists working together to diagnose systemic diseases early based on manifestations through nail changes. This should serve the needs of podiatrists, dermatologists, nail service specialists, cosmetic product developers, and clients who want a safe mix of aesthetics and health for their nails.

Objective: To compare the efficacy, safety, advantages and complications of the silicone ring tourniquet compared to the pneumatic cuff tourniquet in total knee arthroplasty. Methodology: systematic review with meta-analysis, in which a bibliographic search was carried out through the electronic databases: PubMed/MEDLINE and Cochrane Library without language restriction of publications until October 26, 2023. The search terms "tourniquet" AND "pneumatic cuff" AND "knee arthroplasty" AND "orthopaedic surgeries" were used. Results: 292 patients aged between 67 and 74 years were included. Most of the studies presented showed that patients undergoing ischemia with the SRT had less post-operative blood loss, earlier discharge and lower complication rates than other patients. Conclusion: The use of the silicone ring tourniquet resulted in better clinical outcomes than conventional pneumatic tourniquets in TKA.

Prenatal stress has been increasingly recognized as a significant factor influencing fetal brain development, leading to long-term neuroanatomical and psychiatric consequences. This literature review synthesizes current research on the impact of maternal stress during pregnancy on offspring neurodevelopment, focusing on structural brain abnormalities and associated psychiatric disorders. Evidence suggests that prenatal stress disrupts neurogenesis, synaptic plasticity, and hypothalamic-pituitary-adrenal (HPA) axis function, increasing susceptibility to conditions such as autism spectrum disorder (ASD), attention-deficit/hyperactivity disorder (ADHD), schizophrenia, and mood disorders. Understanding these mechanisms is crucial for developing early intervention strategies to mitigate adverse outcomes.

Abstract: Objectives: To evaluate the efficacy and safety of proximal femoral hemiepiphysiodesis (PFH) in the management of hip dysplasia in children with cerebral palsy (CP), by means of a systematic review with meta-analysis.

Methodology: A systematic review was conducted according to the PRISMA guidelines. Clinical studies investigating PFH in children with CP, with or without a control group, were included. The search was carried out in PubMed, Embase, Scopus, Web of Science and Cochrane Library, with no language or date restrictions. The primary outcomes included the progression of femoral migration (Reimers index), the need for subsequent surgery and complications related to the procedure. The quality of the studies was assessed using instruments appropriate to the methodological design, and the data was synthesized using meta-analysis.

Results: Three studies were included, totaling 84 patients. The meta-analysis showed a significant reduction in the Reimers migration index after HPF (mean difference: -5.91%; 95% CI: -10.55 to -1.27; p < 0.01). The rate of need for subsequent surgery was 24% (95% CI: 14%-38%), while the rate of complications was 18% (95% CI: 11%-29%). The overall quality of the studies ranged from moderate to high.

Conclusion: Proximal femoral hemiepiphysiodesis has been shown to be effective in containing femoral migration in children with cerebral palsy, with acceptable rates of complications and reoperations. These findings support its use as a less invasive alternative in the management of hip dysplasia in this population.

The identification of semen is crucial in sexual assault investigations. This article reviews current methods for the forensic identification of seminal stains, encompassing initial detection, confirmatory testing, and DNA analysis. We discuss the principles, applications, and limitations of each technique. A comprehensive approach is essential, starting with initial detection methods like alternate light sources (ALS) to locate potential stains. Confirmatory tests, including microscopic identification of spermatozoa and biochemical assays for prostate-specific antigen (PSA) or semenogelin, provide more specific evidence. DNA analysis, particularly autosomal and Y-STR profiling, offers the highest level of individualization. This review highlights the importance of these techniques in providing evidence for legal proceedings, while also acknowledging factors that may complicate semen detection, such as the absence of spermatozoa or degradation of seminal components.

Asthma is the most common chronic inflammatory disease of the airways, characterized by episodes of obstruction. The aim of the study is to assess the state of the gut microbiome in children with bronchial asthma and to investigate its interaction with Chlamydia pneumoniae and Mycoplasma pneumoniae infections in the course of the disease. Materials and methods. All patients were examined at the Department of Pediatric Allergology of the Tashkent Medical Academy. The study included 14 children with severe, 23 with moderate, and 31 with mild forms of BA. Age distribution: 7–10 years – 23 children (34%), 11–14 years – 20 children (30%), 15–17 years – 25 children (36%). Duration of the disease: 1–3 years — 34 children (50%), 3–6 years — 34 children (50%). Gender distribution: 43 boys (63%) and 25 girls (36%). Results. All children with BA experienced breathing difficulties, mainly at night. In 82.3% of cases, bronchial asthma attacks (shortness of breath, dry cough) occurred 1–3 times per month, lasting 5–10 minutes. In patients infected with M. pneumoniae and C. pneumoniae, significant alterations in the gut microbiota were observed. These changes manifested as a decrease in beneficial bacteria — Bifidobacterium and Lactobacillus. In infected children, the number of Bifidobacterium decreased by an average of 48% (p<0.05), while Lactobacillus decreased by 42% (p<0.01). Additionally, an increase in conditionally pathogenic bacteria — Escherichia coli, Clostridium, and Bacteroides — was detected: Clostridium increased by 35% (p<0.01), Bacteroides by 29% (p<0.05), and E. coli by 33% (p<0.01). These infectious agents contribute to the development of dysbiosis, characterized by a reduction in beneficial microorganisms (Bifidobacterium, Lactobacillus) and an increase in conditionally pathogenic bacteria (Escherichia coli, Clostridium, Bacteroides). Such changes are associated with an enhanced systemic inflammatory response and a higher susceptibility to allergic reactions, which aggravate the clinical course of the disease. Conclusion: Mycoplasma pneumoniae and Chlamydia pneumoniae significantly affect the composition of the gut microbiome in children with bronchial asthma. These infectious agents contribute to the development of dysbiosis, characterized by a decrease in beneficial microorganisms (such as Bifidobacterium and Lactobacillus) and an increase in conditionally pathogenic microorganisms (such as Escherichia coli, Clostridium, and Bacteroides). These alterations in the intestinal microbiota are associated with enhanced systemic inflammatory processes and increased susceptibility to allergic reactions, which in turn aggravate the clinical course of the disease.

The shift of contemporary aesthetic medicine toward non-invasive interventions necessitates not only clinical verification of their efficacy but also a profound understanding of the underlying mechanisms that extend far beyond trivial stimulation of collagen genesis. The aim of the study is the conceptual systematization of molecular determinants (epigenetic and mitochondrial) and the evaluation of the clinical effectiveness of synergistic non-injectable protocols focused on the restoration of dermal homeostasis. The methodological framework of the work is based on a systematic review of scientific literature devoted to the biophysical and cellular foundations of non-injectable therapeutic interventions, as well as on a retrospective analysis of a clinical case study of a multimodal protocol (N=40, DermaReboot Protocol). The obtained data indicate that non-injectable energy-based interventions, in particular photobiomodulation and microcurrent therapy, mediate modification of the cellular response predominantly through non-thermal pathways, including activation of mitochondrial ATP synthesis and key epigenetic regulators (SIRT1, FOXO). Synergistic regimens integrating device-based stimulation (RF, EMS, LED) with topical delivery of signaling molecules (peptides and growth factors) demonstrate objectively measurable clinical effectiveness: there is a statistically significant increase in skin elasticity (on average by +47%) and a pronounced reduction in wrinkle depth (on average by -42%). Rational, intelligent integration of biophysical stimuli and signaling molecules forms a validated and physiologically substantiated strategy of non-injectable dermal regeneration aimed at restoring the homeostatic balance of the skin. The presented work has substantial scientific and practical significance for dermatology researchers and clinicians working in the field of regenerative and aesthetic medicine.

A study conducted from January 2024 to January 2025 at the Tashkent Medical Academy and the 44th Family Polyclinic aimed to assess the impact of breastfeeding on the risk of allergic diseases in children and the mechanisms of its protective effects. A total of 187 children aged 0 to 3 years participated in the study. The primary assessment method was a survey of mothers, along with an analysis of clinical data, including skin manifestations, respiratory symptoms, and food intolerance. Children with identified neurological disorders, congenital anomalies, and severe somatic diseases were excluded from the study. The results showed that breastfeeding for six months or more significantly reduces the risk of developing allergies (p<0.05). The prevalence of atopic dermatitis among breastfed children was 12.4%, whereas among formula-fed children, it reached 34.7%. A similar trend was observed for respiratory allergies: 9.8% in breastfed children versus 27.3% in formula-fed children. A correlation was found between the duration of breastfeeding and a reduction in skin allergies (r=-0.42, p<0.01) as well as food intolerance (r=-0.36, p<0.05). Additionally, children who were breastfed for more than 12 months had lower IgE levels (p<0.05), indicating a stronger immune defense.Conclusion. Breastfeeding, particularly for six months or longer, has a pronounced protective effect, reducing the risk of allergic diseases, including skin, respiratory, and food allergies. These findings confirm the importance of natural breastfeeding as an effective allergy prevention strategy in early childhood.

The specific features of occupational health and safety (OHS) assurance in pharmaceutical manufacturing have been analysed. The regulatory framework and the main risks associated with physical, chemical and ergonomic factors are examined. A systemic set of organisational, technical, sanitary and hygienic measures and socio-economic methods adapted to the particularities of pharmaceutical production is presented. Four practical case studies (evacuation SOP, microbiological air-contamination monitoring, solvent-evaporation prevention, ergonomics on packaging lines) are provided, together with an analysis of outcomes and quantitative indicators.

Gestational diabetes mellitus (GDM) is a disease characterized by hyperglycemia, first detected during pregnancy, but not corresponding to the diagnostic criteria of manifest diabetes mellitus (DM). According to modern concepts, pregnancy is a "diabetogenic factor", since during gestation there is a change in hemostasis of the female body, including carbohydrate metabolism. Hyperglycemia during pregnancy is associated with the development of complications both on the part of the mother and the fetus. In addition, GDM is a risk factor for the development of obesity, type 2 diabetes and cardiovascular diseases in the mother and offspring in the future. According to the International Diabetes Federation, approximately 14% of pregnancies are associated with GDM, resulting in 18 million births per year. All of these children are at risk of developing obesity and type 2 diabetes. The increasing prevalence of this disease, the high probability of adverse pregnancy outcomes for the mother and fetus, and a number of long-term consequences of GDM dictate the need for its prevention by correcting risk factors, timely diagnosis, and effective treatment of the disease.

Objective: To update the treatment and management of rheumatoid arthritis. The secondary objective is to provide the orthopedic medical community with greater confidence in evidence-based treatment. Methodology: Systematic review with meta-analysis carried out in the Prospective Register of Systematic Reviews (PROSPERO)

database ID CRD42024570667. The search was carried out in databases linked to MEDLINE using the terms treatment and rheumatoid arthritis. Results: 1,157 patients were evaluated, of whom 715 were treated with oral methotrexate and 442 with subcutaneous methotrexate. Adverse events resulting from the administration of methotrexate, whether oral or subcutaneous, do occur, in some patients they are more severe and some even give up on this therapy. Among the gastric manifestations, diarrhea was one of the most frequent, as were headaches in neurological manifestations and local symptoms resulting from administration, common to the subcutaneous route. Conclusions: If the oral route is not bearable due to gastrointestinal effects, studies suggest an appropriate switch to the subcutaneous form, to allow continuous use and maintain disease control.

Public water fluoridation, once a celebrated preventive dentistry milestone, is increasingly being reevaluated in light of current scientific, ethical, and policy considerations. While initially justified based on data suggesting systemic benefits from ingestion, current evidence demonstrates fluoride’s primary anticaries mechanism is topical, not systemic. Simultaneously, recent studies have linked fluoride ingestion—even at levels considered safe (0.7 mg/L) by the U.S. Department of Health and Human Services (HHS)—to neurodevelopmental harm, endocrine disruption, and musculoskeletal risks. This article presents a review of the scientific literature addressing fluoride’s mechanisms of action, its systemic risks, and the ethical challenges posed by involuntary mass medication. Based on recent epidemiological findings, policy developments, and bioethical principles, the continued practice of public water fluoridation appears scientifically outdated and ethically untenable.