CELLULOLYTIC ACTIVITY OF BACILLUS SUBTILIS STRAINS OBTAINED FROM DOMESTIC ANIMALS
Kutlieva G.Dj. , Institute Of Microbiology Of The Academy Of Sciences Of The Republic Of Uzbekistan Turaeva B.I. , Institute Of Microbiology Of The Academy Of Sciences Of The Republic Of Uzbekistan Kamolova Kh.F. , Institute Of Microbiology Of The Academy Of Sciences Of The Republic Of Uzbekistan Kuziev M.S. , Department Of Animal Physiology Of Samarkand State University, Uzbekistan Khaidarov D.Ya. , Department Of Animal Physiology Of Samarkand State University, UzbekistanAbstract
Ruminants have a varied population of bacteria, fungus, and protozoa in their rumens. Ruminants may digest plant nutrients thanks to a mechanism of symbiosis with microbes. Due to their high cost, many commercial enzymes for the bioconversion of cellulose-containing materials into simple, easily digestible sugars are hard to come by, which limits their usefulness. Finding bacterial strains that can produce different hydrolytic enzymes - among which the complex of cellulolytic enzymes is of special interest - seems pertinent in this regard.
In the course of research, more than 20 strains of bacteria have been found from the rumen of domestic animals. To identify producers of cellulolytic enzymes, we screened obtained aerobic spore-forming bacteria of the genus Bacillus. As a result, we discovered that 6 strains of Bacillus subtilis have the ability to degrade carboxymethylcellulose.
Keywords
Microorganisms, bacilli, cellulose
References
Bach A. Ruminant nutrition symposium: Optimizing performance of the offspring: Nourishing and managing the dam and postnatal calf for optimal lactation, reproduction, and immunity J. Anim. Sci., 90 (2012), pp. 1835-1845, https://doi.org/10.2527/jas.2011-4516
Bedford, M., and Partridge, G. (2010) Enzymes in farm animal nutrition, CABI, UK.
Bacic, A., Fincher, G., and Stone, B. (2009) Chemistry, Biochemistry and Biology of (1–3) β-Glucans and Related Polysaccharides, Academic Press, N.Y
J. Laporta, F.C. Ferreira, V. Ouellet, B. Dado-Senn, A.K. Learn more about the role of rumen microorganisms and their impact on the host’s performance and health. Journal of Dairy Science (https://www.sciencedirect.com/journal/journal-of-dairy-science) Volume 103, Issue 8 (https://www.sciencedirect.com/journal/journal-of-dairy-science/vol/103/issue/8), August 2020, pp. 7555-7568.
Bezborodov A.M., Astapovich N.I. Secretion of enzymes in microorganisms. - Moscow: Nauka, 1984. – p.72.
Bilay V.I. Pidoplichko N.M., Taradiy G.V. Cellulolytic properties of mold fungi and principles of selection of active cellulase producers // Enzymatic cleavage of cellulose. – M.: Nauka, 1967. – pp. 37–45.
Bisswanger H. Practical enzymology, M., YuINOM. Knowledge Lab. 2010.
Polygalina G.V., Cherednichenko V.S., Rimareva L.V. Determination of enzyme activity. Directory. M., DeLi print, 2003.
Sinitsyn A.P., Chernoglazov V.M., Gusakov A.V. Methods for studying and properties of cellulolytic enzymes. Results of science and technology, ser. Biotechnology. VINITI, v.25, 1993.
Kaschaev A., Petrenko A., Kalashnikov A. Feed additives based on living cultures of microorganisms // Poultry farming. - 2006. - No. 11. - pp. 43-45.
Klesov A.A., Rabinovich M.L. Enzymatic hydrolysis of cellulose // Biological chemistry. Results of science and technology. - Moscow, 1982. - T. 8, No. 11. - pp. 1490-1496.
Preobrazhensky S.N. Pharmacodynamic bases and prospects for the use of enzyme preparations in animal husbandry // Wind. s.kh. alive. - 2006. - No. 1. - pp. 71–75.
Rabinovich M.L., Chernoglazov V.M. Klesov A.A. Classification of cellulases, their prevalence, multiple forms and mechanisms of action of cellulases. "Results of science and technology VINITI". Biotechnology. - 1988. - T. 11. - pp. 1-224.
Rukhlyadeva A.P., Polygalina G.V. Methods for determining the activity of hydrolytic enzymes. - Moscow: Easy. and food. industry, 1981. - 288 p. 48 ISSN 0201-8462. Microbiol. journal, 2009, Vol. 71, No. 5.
Ryadchikov V., Petrenko A., Radul A. Bacellus in feed for chickens and rearing young animals // Poultry farming. - 2005. - No. 1. - pp. 23–24.
Sinitsyn A.P., Mitkevich O.V., Kalyuzhny S.V., Klesov A.A. Study of synergism in the action of enzymes of the cellulase complex // Biotechnology. - 1987. - No. 1. - pp. 39-46.
Ushakova N.A., Belov L.P., Varshavsky A.A., Kozlova A.A., Kolganova T.V., Bulygina E.S., Turova T.P. Cleavage of cellulose under nitrogen deficiency by bacteria isolated from the intestines of herbivorous vertebrates // Microbiol. magazine - 2003. - 72, No. 3. - pp. 400-406.
Fumiyasu F.,Toshiaki K., Koki H. Purification and Properties of a Cellulase from Alkalophilic Bacillus sp. № 1139 // J.of Gen. Microbiol. – 1985. – 131. – pp. 3339-3345.
Article Statistics
Copyright License
Copyright (c) 2022 The American Journal of Veterinary Sciences and Wildlife Discovery

This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors retain the copyright of their manuscripts, and all Open Access articles are disseminated under the terms of the Creative Commons Attribution License 4.0 (CC-BY), which licenses unrestricted use, distribution, and reproduction in any medium, provided that the original work is appropriately cited. The use of general descriptive names, trade names, trademarks, and so forth in this publication, even if not specifically identified, does not imply that these names are not protected by the relevant laws and regulations.