Articles
| Open Access |
DOI: Genetic identification of yellow rust disease resistance in soft wheat (Triticum Aestivum l.) Samples using DNA markers
Durdona Shokirova , Uzbek Academy of Sciences, Research Institute of Plant Genetics and Experimental Biology, Tashkent, Uzbekistan Xurshid To‘raqulov , Uzbek Academy of Sciences, Research Institute of Plant Genetics and Experimental Biology, Tashkent, Uzbekistan Toxir Bozorov , Uzbek Academy of Sciences, Research Institute of Plant Genetics and Experimental Biology, Tashkent, Uzbekistan Sodir Meliev , Uzbek Academy of Sciences, Research Institute of Plant Genetics and Experimental Biology, Tashkent, Uzbekistan Shohida Ibragimova , Uzbek Academy of Sciences, Research Institute of Plant Genetics and Experimental Biology, Tashkent, Uzbekistan Fazlidin Meliqo‘ziev , Uzbek Academy of Sciences, Research Institute of Plant Genetics and Experimental Biology, Tashkent, Uzbekistan Abdurauf Dolimov , Uzbek Academy of Sciences, Research Institute of Plant Genetics and Experimental Biology, Tashkent, Uzbekistan Bexruz Ochilov , Uzbek Academy of Sciences, Research Institute of Plant Genetics and Experimental Biology, Tashkent, Uzbekistan Sojida Murodova , Uzbek Academy of Sciences, Research Institute of Plant Genetics and Experimental Biology, Tashkent, Uzbekistan Ilham Aytenov , Uzbek Academy of Sciences, Research Institute of Plant Genetics and Experimental Biology, Tashkent, UzbekistanAbstract
This study assessed the genetic polymorphism of wheat samples in relation to yellow rust disease resistance through DNA markers genetically linked to this trait. According to the analysis, the markers Xgwm140 (PIC = 0.72) and Xgwm340 (PIC = 0.53) exhibited the highest levels of polymorphism, playing a significant role in the identification of yellow rust-resistant alleles, with 305 and 220 base pairs, respectively. Phylogenetic analysis revealed genetic diversity among the genotypes and indicated that resistant genotypes tended to cluster into distinct groups. The findings of this study provide a reliable tool for identifying resistant genotypes, which can be effectively utilized in the selection process during wheat breeding programs aimed at enhancing resistance to yellow rust disease. This version reflects a more detailed and formal scientific tone, maintaining the essence of your original text while providing further clarity on the methods and outcomes. Let me know if you need any further adjustments. In 2024 field trials, wheat varieties were tested for yellow rust resistance using molecular markers for Yr genes. Varieties with Yr5 and Yr15 showed full resistance, while those with Yr6, Yr9, Yr7, and Yr27 were susceptible. Yr62 alone was weak but enhanced resistance when combined with other genes. Yr5 and Yr15 were identified as the most effective for breeding resistant varieties.
Keywords
Wheat (Triticum aestivum L.), DNA markers, PCR
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Copyright (c) 2025 Durdona Shokirova, Xurshid To‘raqulov, Toxir Bozorov, Sodir Meliev, Shohida Ibragimova, Fazlidin Meliqo‘ziev, Abdurauf Dolimov, Bexruz Ochilov , Sojida Murodova, Ilham Aytenov
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